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Alginate (freeze dried, extra pure)

Protocol number:	M002242-1
Industry:	Biology
Feed Size:	< 3mm
Desired Fineness:	< 20µm
Quantity:	20g
Recommendation:	For grinding, we recommend using a planetary mill like Planetary Mono Mill PULVERISETTE 6 classic line and bowls made of sintered corundum.

Result 1

	Planetary Mono Mill PULVERISETTE 6 classic line main disk speed: 600 rpm
Equipment:	250 ml grinding bowl made of sintered corundum (Al2O3) + 15x 20 mm Ø Al2O3 grinding balls
Feed quantity:	20 g (=60 ml)
Feed Size:	< 3 mm
Additive:	+ 70ml ethanol
Grinding time:	30 min
Final fineness:	91,1%<20 µm; 4,7%< 1 µm
Comments:	To avoid overpressure, we ground the sample in steps of 5 minutes, followed by a programmed pausing time of 10 minutes. After several cycles, the outside temperature of the bowl should be checked (remain below 70°C); grinding time or programmed pausing time might be readjusted afterwards. By using sinter corundum, higher wear off is expected to be found. After totally 30 minutes of grinding time, already 91,1% of sample use to be < 20µm as desired. Longer grinding time, or a change of ball diameter (e.g. 10mm Ø) is still possible for further size reduction.